FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_50110001-2-mss.3520000003c868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_50110001-2-mss.3520000003c868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6478088
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG213150.0137.2107245
GGGGGGG384250.071.14532235-239
GGCGGGG65150.065.14145245
CGAGGGG35950.053.893257245
GTAGGGG51100.050.55349245
CGAGCCC23850.050.288532245
CGCGGAC8307.460108E-444.462708245
CCACCCC249800.042.104298245
GGCGGCG20008.4764906E-1041.517056245
AGAGGGG125100.040.562004245
CGGGGGG73800.040.54573235-239
GGAGGGA171350.040.382313245
AGGGGGG137500.040.11906235-239
CGACCCG9600.001526247238.44172245
GGCGGGA36200.038.229332245
GAAGGGG157900.037.394855245
CGAGCGG10150.002006539136.35867245
GGAGGGC82300.035.872707245
CGACCCC28351.8189894E-1135.797577245
GGAGGAA170600.034.611065245
Produced by FastQC (version 0.11.2)