FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_41442-mss.3520000003c84f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_41442-mss.3520000003c84f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6110665
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG174000.0133.20555245
GGCGGGG56500.089.89015245
GGGGGGG345350.067.15393235-239
CGAGGGG29650.052.94465245
CGACCCC23800.050.438744245
GGCGGGA29150.044.349358245
AGGGGGG116150.042.673428235-239
GAAGGGG140600.040.0629245
GGAGCGG26709.094947E-1238.043396245
GTAGGGG42500.036.93668245
AGAGGGG108750.036.51212245
GGAGGGC74200.034.845924245
CGGGGGG66050.033.96959235-239
CCACCCC201400.033.92893245
GGAGGAG147350.033.84087245
GGAGGGA139350.033.79567245
GGAGGAA148650.033.544914245
GCAGGGG100050.032.303444245
CCGGGGG48150.030.924095235-239
GGCGGCG18103.0404444E-530.610508245
Produced by FastQC (version 0.11.2)