FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_40825-mss.3520000003c832.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_40825-mss.3520000003c832.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596289
Sequences flagged as poor quality0
Sequence length251
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGG11350.081.312256245
CGACGGG3550.001446902977.99104245
GGGGGGG57650.068.536224235-239
GGAGGGG36700.062.86744245
GCACCCC13159.513315E-1056.14564245
CGAGGGG7454.3640568E-449.55136245
GGAGGGT13105.858601E-849.314953245
GGAGGGC15203.43789E-948.573368245
GTAGGGG9953.245269E-546.376587245
AGGGGGG25900.037.744987235-239
CCACCCC48550.036.11738245
GGAGGAC16651.7175811E-533.257442245
GGAGCCC11150.003169779733.108307245
CCGCCCC11156.801747E-733.108307245
GGAGGAA35357.2759576E-1231.328793245
AAGGGGG22900.031.12794235-239
ACGGCGA3050.00816084230.258823245
TGGGGGG17050.029.862999235-239
CGGGGGG13100.029.539293235-239
GGAGACC13200.00723638727.966486245
Produced by FastQC (version 0.11.2)