FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_35011-1-mss.3520000003c85c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_35011-1-mss.3520000003c85c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714360
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG204300.0149.7286245
GGCGGGG66650.077.88379245
GGGGGGG388600.074.1488235-239
CGAGGGG34400.056.58744245
CCACCCC222900.044.497223245
GGAGGCG56850.042.393776245
CGAGCCC19907.694325E-1041.92264245
AGGGGGG134650.040.832684235-239
GGCGGGA31800.040.80939245
GTAGGGG47350.039.153378245
GGACCCC57900.038.423058245
GGAGGAG161750.038.39633245
GGAGGGA158050.038.122208245
GAAGGGG153900.037.945576245
CGGGGGG73000.037.65836235-239
CGACCCC27651.2732926E-1136.877098245
CCGCGGG15205.1399184E-736.590374245
GGACGGG39700.035.023533245
GTCGGGG18809.695505E-734.514328245
AGAGGGG120550.031.526506245
Produced by FastQC (version 0.11.2)