FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_35010-mss.3520000003c7e0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_35010-mss.3520000003c7e0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315194
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG141150.0122.431015245
GGCGGGG43050.076.46098245
GGGGGGG235100.071.095116235-239
GTAGGGG29800.058.297226245
CGAGGGG22900.055.89888245
GGCGGAC11407.670939E-540.102894245
AGGGGGG89650.039.9034235-239
GGAGGGT47550.038.458294245
GGCGGAA16654.1677777E-738.44097245
CGGGGGG44750.038.38953235-239
GGAGGAG111700.036.835785245
CCACCCC168150.036.43246245
CCGGGGG35850.036.08093235-239
GAAGGGG97000.035.81974245
GGACCCG17907.347735E-735.756546245
GGAGCCG18208.368297E-735.167156245
AGAGGGG91900.034.822754245
GGAGGAA115650.033.996433245
GGAGGGC54600.033.492527245
GCACCGT11950.004665252330.605724245
Produced by FastQC (version 0.11.2)