FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_35005-1-mss.3520000003c825.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_35005-1-mss.3520000003c825.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3832
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTT50.00190308771940.5245
CCAACGA50.00190308771940.5245
AGAGGTC50.00190308771940.5245
CAGAGCC100.0076103895970.25245
GTACACT100.0024456347204.263156
TTCTTCA150.008224971136.175459
GAAGGAA200.00811582136.17543225-229
Produced by FastQC (version 0.11.2)