FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_34344-1-mss.3520000003c818.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_34344-1-mss.3520000003c818.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340322
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG212250.0115.033485245
GGGGGGG365750.070.32562235-239
GGCGGGG69100.065.33474245
CGACGGG16151.8189894E-1257.04973245
CGAGGGG40350.050.23487245
GTAGGGG52600.038.535683245
CGGGGGG79450.037.787354235-239
AGGGGGG140050.037.659016235-239
GGAGGCG61250.036.102245
CGACCCC32201.8189894E-1234.336143245
CCACGCG16801.8550554E-532.90547245
GAAGGGG163900.032.604324245
GGAGGAG178350.032.545696245
GTAGGCG17152.1360021E-532.233932245
CCACCCC267800.031.99621245
AGAGGGG136350.031.759148245
GGAGGGC82700.031.194548245
ATAGGCG17752.7019321E-531.144333245
CCGGGGG60750.030.052107235-239
AGCGGGG40450.029.610857245
Produced by FastQC (version 0.11.2)