FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_34098-mss.3520000003c89f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_34098-mss.3520000003c89f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204743
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG102650.085.70432245
GGGGGGG123800.062.49895235-239
GGCGGGG32700.054.905754245
GTAGGGG28450.044.17549245
CGAGGGG23551.2914825E-1038.119286245
GGAGGCG32753.6379788E-1232.893158245
GAAGGGG81200.032.06104245
CGGGGGG34850.030.065105235-239
AGGGGGG65250.028.10108235-239
CCGGGGG28350.027.718756235-239
GGCGGCG13100.00799414227.410967245
CTAGGGG23106.1991686E-627.203306245
GTCGGGG9952.1307886E-427.066605245
CCACCCC153600.026.884518245
CGACGCC16758.173103E-426.797287245
GTAGGGC17650.001109301325.430853245
TGAGGGG42601.03682396E-1025.28758245
AGAGGGG74700.025.236803245
CGACCCC24951.1273974E-525.186226245
GGAGGAA108950.024.718927245
Produced by FastQC (version 0.11.2)