FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_34042-1-mss.3520000003c8fc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_34042-1-mss.3520000003c8fc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6837448
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG189450.0107.083115245
GGGGGGG282300.065.633865235-239
CGAGGGG40100.045.783493245
GGCGGGG64050.044.428936245
CGACGGG17551.2945748E-841.844288245
GTAGGGG48900.037.544334245
AGGGGGG126400.035.803535235-239
CGGGGGG66900.035.491787235-239
GAAGGGG146950.031.858397245
GGAGGCG59700.030.7524245
AGAGGGG128550.029.991663245
CCGGGGG54800.029.626307235-239
GAAGGCG32553.2941898E-928.201508245
CGACCCC40404.0017767E-1127.266111245
CCACCCC272550.026.944315245
GGAGGAG173150.025.977472245
GGAGGGA170100.025.903606245
GCAGGCG42758.185452E-1125.767275245
GGAGGGC84000.025.134594245
GGAGGAA181050.024.336943245
Produced by FastQC (version 0.11.2)