FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_34012-1-mss.3520000003c8ef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_34012-1-mss.3520000003c8ef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7086222
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG216900.0124.1539245
GGCGGGG81450.072.71362245
GGGGGGG393700.065.7586235-239
GTAGGGG58550.044.25456245
CGAGGGG42250.041.61537245
GGAGGCG67800.039.581768245
GGAGGGA180700.037.896618245
CGGGGGG86250.037.165436235-239
CCACCGG42450.037.05938245
AGGGGGG145700.036.574604235-239
CCACCCC301050.035.34973245
GGCGGAA32051.8189894E-1234.648155245
GAAGGGG160450.032.874718245
GTACGGG14502.9423577E-431.910152245
AGAGGGG140700.030.912252245
CCGGGGG70050.029.729715235-239
TTAGGGG53450.027.701237245
GGTGGGG147550.027.595634245
GGAGGGC94000.027.564941245
GGAGGAG186500.026.298073245
Produced by FastQC (version 0.11.2)