FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30749-mss.3520000003c7d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30749-mss.3520000003c7d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3634229
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG93250.098.7963245
GGCGGGG27850.075.330475245
GGGGGGG132800.065.00488235-239
GTCGGGG9302.3382341E-549.04053245
CGAGGGG18554.5110937E-1044.255444245
GTAGGGG28500.041.607018245
GGCGGAA14356.7405217E-638.13884245
AGGGGGG60300.038.131847235-239
CGGGGGG28700.037.95003235-239
AGAGGGG64600.035.300076245
GGACCCC29002.7284841E-1134.59894245
GGAGGCG29202.910383E-1134.36196245
CCGGGGG22200.033.61623235-239
GAAGGGG69550.032.787704245
GTAGGGC14603.33077E-431.238148245
GGAGGGA79700.030.901073245
GGACGGC12200.00522236429.906687245
CCGCGCC12350.005543830329.543447245
GGACGGG24902.9612602E-729.30615245
CGAGGGC12500.005880745629.188925245
Produced by FastQC (version 0.11.2)