FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30521-mss.3520000003c8ac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30521-mss.3520000003c8ac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5903586
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG154000.0136.38686245
GGGGGGG269000.069.885544235-239
GGCGGGG51700.064.79126245
CGAGGGG29650.054.960804245
GTAGGGG39800.047.768486245
GGCGGTT12652.9873481E-642.94039245
CCACCCC211200.042.008526245
GAAGGGG121200.040.33633245
CGCGGGG15750.040.236736245
CGCGGGA9150.00132273639.577118245
CGGGGGG56100.038.23551235-239
AGGGGGG108050.037.64395235-239
CCACGGG31501.8189894E-1234.488632245
GGAGGCG46400.033.169292245
GGAGGGA138500.032.68327245
CCGGGGG43350.032.6763235-239
GGACCCC48450.031.765842245
GGAGGGC68500.031.719473245
GGACGCG11950.00489834530.303818245
CGACCCC28252.2855602E-828.842262245
Produced by FastQC (version 0.11.2)