FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30484-1-mss.3520000003c8d2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30484-1-mss.3520000003c8d2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7490490
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG253350.0117.21771245
GGCGGGG88800.066.67702245
GGGGGGG402600.065.84484235-239
CGAGGGG45150.051.22611245
GTAGGGG60250.042.994225245
CGACCCG13003.1063937E-642.698933245
CGACCCC44450.039.544945245
CCACCCC330600.038.89745245
CGCGGGG26350.038.620796245
GGAGGGA199550.036.161964245
GTCGGAA15951.2644219E-534.80164245
GTAGGCG18759.646974E-734.538692245
AGGGGGG160850.034.17647235-239
GAAGGGG176000.034.167236245
GGAGCGG43750.033.83382245
CGGGGGG87550.033.255573235-239
GGACCCG36850.032.63736245
CTAGCGC11400.003498957332.461178245
GTCGGGG24802.5310874E-729.843342245
CGAGGGC28052.2449967E-829.683754245
Produced by FastQC (version 0.11.2)