FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30423-1-mss.3520000003c8c5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30423-1-mss.3520000003c8c5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6423212
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG192100.0128.31384245
GGGGGGG305750.064.42964235-239
CGAGGGG32350.061.628323245
GGCGGGG66750.055.66274245
GTAGGGG46750.042.645485245
CGGGGGG69950.038.61396235-239
AGGGGGG124900.035.93439235-239
TTAGGGG46350.035.192875245
AGAGGGG123550.033.00667245
CCGGGGG57450.032.522076235-239
CGAGCGG11650.004304979531.114733245
CCACCCC280700.030.9928245
GGTGGGG124900.030.473255245
GTCGGGG17853.144081E-530.461061245
GGAGGGC81050.030.188583245
GAAGGGG142350.029.28413245
GGAGGCG58950.029.207998245
GAAGGCG29804.9383743E-827.368958245
AGCGGGG33550.027.010927245
GGAGGGA162450.026.776482245
Produced by FastQC (version 0.11.2)