FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30245-mss.3520000003c7c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30245-mss.3520000003c7c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074704
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG131900.0130.99623245
GGCGGGG37950.068.17234245
GGGGGGG226250.066.40372235-239
CGAGGGG24650.059.974277245
GTAGGGG32050.054.77565245
GTCGGGG11851.6554368E-646.78373245
GAAGGGG96150.042.282955245
GGAGGCG38250.041.065723245
TTAGGGG31450.038.193077245
AGGGGGG86450.037.256798235-239
CCACCCC157850.035.706493245
GGCGAAA22651.1542397E-732.63501245
GGTGGGG84900.031.561111245
CCGGGGG30950.030.956415235-239
CGAGGGC15203.912769E-430.394035245
CGAGGCC18503.5141165E-529.966877245
GGAGGGC52650.029.83407245
GGAGGAA122000.029.537024245
GGGGGCG30500.028.739689235-239
GCACGGG19304.6906884E-528.724726245
Produced by FastQC (version 0.11.2)