FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n02_30230-1-mss.3520000003c8b8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n02_30230-1-mss.3520000003c8b8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195071
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGGG178600.0115.22687245
GGCGGGG58200.081.00088245
GGGGGGG288500.070.39655235-239
CGAGGGG31850.054.082092245
GGCGGTT13801.02119884E-745.986282245
GTAGGGG46450.042.938446245
GGAGGCG48900.038.93317245
CGACCCC32850.038.636875245
GAAGGCG23501.1459633E-1038.578156245
AGGGGGG110100.036.67152235-239
GGAGGGC74000.035.528397245
CCACCCC241050.034.97721245
AGCGGGG29800.033.46461245
CGGGGGG66400.032.694813235-239
GGAGGGA146850.032.10249245
TTAGGGG42450.032.034866245
TCGCCCC17853.1351665E-530.473503245
TGAGGGG68500.030.440136245
GAAGGGG132700.030.060144245
GGACCGC15354.633522E-429.530512245
Produced by FastQC (version 0.11.2)