FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_nw2-mss.3510000003c7f0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_nw2-mss.3510000003c7f0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46442919
Sequences flagged as poor quality0
Sequence length251
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC295000.018.021929245
GTATATA612250.017.593251
GTATAGA394950.016.8167921
GTATAGG279300.016.5846711
GTATTAG409700.016.0917131
GTGTATA559400.015.1151551
TATACTG430800.014.8717425
GTATAAG386200.014.4690011
GTGTAGG387450.013.8846461
GTGTAAG443550.013.7309261
TCTATAC405200.013.2726923
GTATTAT472800.013.0628831
GTATTAA446750.012.9468281
TATACAG506900.012.9049065
TACACTG575050.012.86675
GTATAAT411600.012.77226451
GTATATG544750.012.6422691
CTATACT390100.012.5294734
GTGTAGA488100.012.4274611
ACTGATC554850.012.0102928
Produced by FastQC (version 0.11.2)