FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_nw1-mss.3510000003c7bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_nw1-mss.3510000003c7bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43169337
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA612050.016.7577861
GTATATA720000.016.7131021
TATACTG464100.015.62601955
GTATTAG427950.015.0902241
ACTGATC584250.014.15268
GTATAGG256400.013.9076281
GTATAGA416650.013.6466071
GTGTAGG338700.013.3140661
GTGTAGA475000.013.2085291
GTATAAG402450.012.6665841
TATATAC759450.012.6467153
GTATAAT480950.012.6119821
TATACAG538400.012.331975
GTGTAAG460450.012.2154171
GTATTAT545800.012.10133651
GTATTAA520600.012.0751151
GTACTAG213900.011.9732921
ATATACT631200.011.7998324
TACACTG570450.011.5317435
GTATATG591200.011.5244031
Produced by FastQC (version 0.11.2)