FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_50330665-2-mss.3510000003c885.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_50330665-2-mss.3510000003c885.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6354890
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA121850.015.9891091
GTATAGA57500.015.3432481
ACTGATC97050.014.7679418
TATACTG71400.014.7549155
GTATAGG37000.014.5714781
GTGCTAG57450.014.5034581
TACACTG101350.013.5372595
TCTATAC57300.013.4695523
GTGTATA108950.013.3835841
TCCTATA76750.013.2487142
TATATAC125050.013.2256593
GTCCTAC38950.013.212791
GTATTAT77000.012.8898231
CCTATAC44800.012.5790263
CTATACT65300.012.5689234
GTATTAG66200.012.4013641
CTACACT84600.012.1631314
ATATACT92800.012.1444064
GTGTAAG72950.012.0937191
GTATTAA67900.012.0908741
Produced by FastQC (version 0.11.2)