FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_50330665-1-mss.3510000003c878.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_50330665-1-mss.3510000003c878.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6597542
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG42750.018.6327271
GTATTAT79400.016.6687281
TATACTG70600.016.4840285
GTATATA115150.016.1762851
ACTGATC93650.014.3885228
TATATAC122200.014.3358173
GTGTATA102650.014.2065031
GTATATG91550.014.1888331
GTATTAG59200.014.0762311
TCTATAC58350.014.0666793
TATACAG85750.014.0002695
GTGTAGA71750.013.1512791
CGCGACC7703.93389E-412.727132245
ATACTGT106400.012.6647186
GTATAAG60000.012.6630881
GTCTTAG60250.012.6105431
GTCCTAG53800.012.5279371
GTATACA79450.012.4936771
CTATACT65550.012.3343324
GTATAGA60100.012.0303071
Produced by FastQC (version 0.11.2)