FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_50110001-2-mss.3510000003c86b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_50110001-2-mss.3510000003c86b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6478088
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67300.017.1101655
GTGTAGG46700.016.530621
GTGTATA106200.016.3843521
GTATAGG36700.016.3604581
GTATTAG58650.015.669631
GTATAGC31750.015.437671
GTATATA119700.015.3554511
TATACAG76450.014.7418425
CTATACT59700.014.363664
GTACGGA23150.014.2915141
GTATAAG55150.014.2200121
TCTATAC60500.013.9717863
ACTGATC104050.013.539128
TATATAC128400.013.4527873
TCCGTAC20950.013.4493713
CCTATAC44050.013.3491473
GTGTAGA71000.012.9439961
GTGCGCG7603.5137008E-412.8985781
GTATAGA57150.012.8647251
GTGTTAG72500.012.6761891
Produced by FastQC (version 0.11.2)