FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_41442-mss.3510000003c842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_41442-mss.3510000003c842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6110665
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT8251.8189894E-1120.78769
CCTATAC48100.019.6115283
ACTCGCG9659.094947E-1219.0411958
GTCCTAG44450.017.9178371
CTATACT71450.017.6592564
TATACTG83150.017.3842965
GTCCTAA51000.016.3373931
TCCTATA79750.016.1296312
GTATAGG33650.016.021811
CCCTATA43900.015.6274942
GTATATA144600.014.659591
ACGCTAG8503.894771E-614.4153461
CTCCTAT84500.014.3556381
TCTATAC66050.014.2818263
GTCCTAC32750.014.2172741
CCCTAGA66350.014.2172512
CCTAGAC63300.014.12813953
CCCCTAG42500.014.1270391
ATGCTAG62650.014.0817111
GTGCTAC37550.014.0314481