FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_40825-mss.3510000003c835.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_40825-mss.3510000003c835.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1596289
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGT2101.2168803E-534.999849
ACTCGCG2352.6269643E-531.2764518
CGCGCTA350.007213060327.99987220-224
TCCGTAC3758.087063E-726.1332138
CTGTCGC6807.4578566E-1021.617559
GTGCTAG13350.020.1903361
GAGCCGT3100.00481148419.7579736
GTGTAAG16600.019.1896651
CCGTACC3908.1570377E-418.8460679
CCTATAC12600.018.4727153
GTGCTAC9701.8007995E-1017.68311
GTGTAGG10603.45608E-1117.3375421
GCTATAC10603.45608E-1117.335373
GTCCTAT12201.8189894E-1217.0722681
TCTGTCG7951.0480471E-716.9496088
TGCTAGG16200.016.6362462
CTTACAC20800.016.4908263
CCCTATA11901.0913936E-1116.4710272
TGCACTG30500.016.06555
CACTCGC5403.9164216E-415.8795577
Produced by FastQC (version 0.11.2)