FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_35011-1-mss.3510000003c85f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_35011-1-mss.3510000003c85f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5714360
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT21100.020.3197218
GATCGTT23700.018.6074289
CCGTACC18300.018.0736064
GTATTAT63850.017.655991
TATACTG60400.016.8334565
GTATATA100150.016.0282291
GTGTATA91350.015.9625991
TATACAG69350.015.72083955
TATATAC107600.015.2561043
GTATTAA54900.014.9543521
ACGGACC26400.014.848351245
AGGATCG28100.014.821937
ACTGATC96100.014.7865468
TCCGTAC20100.014.6273733
GTATTAG54150.014.2563151
GTATAAT57850.013.9799551
CGTACCA20500.013.7437785
TAGACAG206100.013.6109885
CTAGGAC47300.013.4676983
ATATACT79350.013.4308814
Produced by FastQC (version 0.11.2)