FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_35010-mss.3510000003c7e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_35010-mss.3510000003c7e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4315194
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG50700.018.3629995
GTGTATA71800.016.896811
GTATTAG44600.015.9362861
GTATACG7801.5920687E-615.7108381
GTATATA83350.015.5845491
ACTGATC65750.015.09114558
CCGTACC11652.419256E-914.7210914
TACACTG64900.014.533965
TATATAC85050.014.4043543
CTATACT46150.014.3337564
TCTATAC43900.014.2322573
CACTACG10451.5089245E-714.0685142
ACTACGC9659.4412644E-713.964763
GTGTAGA46900.013.8483171
TACACAG99200.013.8307055
ATGCCGC11704.0545274E-813.61117245
GTACTAG21700.013.5533131
GTATAAG39350.013.3911451
GTATTAT58250.013.2537451
GTATAGA40850.013.1994121
Produced by FastQC (version 0.11.2)