FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_34344-1-mss.3510000003c81b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_34344-1-mss.3510000003c81b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340322
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATATA109850.018.5172961
ACTGATC106050.017.5576138
TATACTG68900.016.3570355
GGATCGT23700.016.0230758
GTCGTAG7651.3013141E-616.0179941
TATACAG79500.015.4088015
GTCCTAG49600.014.8231041
TGCACTG161400.014.8001815
GTATAGA56000.014.6607541
GTGTATA103900.014.624321
CTGATCA119150.014.5991259
GATCGTT24850.014.2956579
GTACGGA19900.014.1626431
CTGTCGC36000.013.9512759
TAGGTCG6150.001050689513.9430875
GCACTGA131950.013.740056
AGGATCG31600.013.5680337
GTGTAGA67300.013.473681
GTATTAG62250.013.1887911
TCTGTCG39050.013.1753078
Produced by FastQC (version 0.11.2)