FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_34098-mss.3510000003c892.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_34098-mss.3510000003c892.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4204743
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG51300.017.9091535
GTATAGA38700.016.7802871
GTATATA78500.016.7012351
GTGTATA69150.016.6559771
TACCCGT10408.60382E-915.3123275
GTATTAG43200.015.032341
TACACTG60500.014.7808245
TAATACT55750.014.7218064
GTATTAA48500.014.6528021
GACTGCG8453.6728816E-614.4968787
ATACTGT76000.014.1840516
GTATAGG24350.014.0894341
GTATAGC20900.014.0701721
GTCTTAG37600.014.0124831
CTATACT40800.013.8111174
TATATAC84800.013.7236373
ATATACT66750.013.580374
CGTTTCG8204.443092E-513.4449689
ATACCCG11002.8239992E-713.3634844
GTCCTAC22950.013.3472491
Produced by FastQC (version 0.11.2)