FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_34042-1-mss.3510000003c8ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_34042-1-mss.3510000003c8ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6837448
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT27750.016.7745428
ACTGATC124500.016.037938
GTGTATA108950.015.85861
GATCGTT30900.015.8573839
CGCCGAA4700.002846010515.638422245
GTATATA116650.015.5471191
GTGTAGG53850.015.2462161
GTATAGG42100.014.8443451
GTATTAG65200.014.6595281
GTATAAG57900.014.6030461
AGGATCG33600.014.5832377
GTATTAT74750.014.425951
CTGATCA131150.014.29068859
TATACAG84900.013.70721155
TATACTG74300.013.6842965
CTACACT82050.013.5861384
GTAGGAC39250.013.4208823
GTACTAG34200.013.25711
GTGTAGA72300.013.0504471
TCTATAC68100.012.9520243
Produced by FastQC (version 0.11.2)