FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_34012-1-mss.3510000003c8e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_34012-1-mss.3510000003c8e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7086222
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG41450.017.4426041
CCTATAC52650.016.5206573
ACTGATC119850.016.2513588
CCCTATA49200.015.6872152
GTATTAG69650.015.3067141
GTATATA130850.015.077731
GTGTATA114700.014.9571361
TATACTG75600.014.5833555
TATACAG92000.014.1141525
GTATTAT77700.014.0363051
CTATACT70100.013.9800494
CTAGTAC37700.013.9731553
GTCCTAC40500.013.9183141
GTATAGA61900.013.8577011
GTGTAGG54850.013.8515661
GTGCTAC46800.013.6157431
CTGATCA134050.013.3418679
CCGTACC17651.8189894E-1213.18708245
TACACTG112250.012.8775245
GTATAAG63950.012.6469911
Produced by FastQC (version 0.11.2)