FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30749-mss.3510000003c7d6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30749-mss.3510000003c7d6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3634229
Sequences flagged as poor quality0
Sequence length251
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA37900.020.690521
GTATATA84550.017.1001171
CGTACCC5753.2744618E-517.043673
TATACTG49150.016.698845
GTGTAGG31100.016.1530341
GTGTATA65050.016.0104051
GCGTAGA7251.3880828E-515.2102081
TATATAC85900.015.1165873
GTATTGG34650.014.8517191
TACACTG55950.014.4503675
GTATTAG38200.014.4337711
CCTATAC24000.014.2918293
GTATAAT49050.013.9887731
GTATTAT57000.013.9723391
TATACAG51100.013.9040765
GTATAGT34050.013.6740491
ACTGATC58400.013.2146848
CCCTATA25100.013.177442
GTGTAGA42500.012.97341251
GTACTGG37900.012.9315751
Produced by FastQC (version 0.11.2)