FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30521-mss.3510000003c8af.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30521-mss.3510000003c8af.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5903586
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG57950.018.8187371
TATACTG67600.018.1211515
GTATTAT68500.017.3514211
GTATATA107100.015.1021351
GTATAGA54450.015.0775521
GTGTATA97200.014.8754341
GTATTAA69100.014.5408471
GTATAAT63500.014.4724291
GGATCGT20550.014.3064528
TCGGGCG5350.00673009513.738205245
GCTATCG7202.2296506E-413.6109988
ATTATAC81250.013.5698083
GATCGTT22250.013.2133759
ATATACT87150.012.931624
TATATAC115600.012.9287513
ATACTGT93650.012.6880956
GTCTATA48900.012.5289611
GTATAGG36250.012.5068411
TATTCCG31400.012.4839735
GTGTTAG71700.012.4754911
Produced by FastQC (version 0.11.2)