FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30484-1-mss.3510000003c8d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30484-1-mss.3510000003c8d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7490490
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG72350.018.4614371
TATACTG78600.017.455335
GTATATA130600.016.2323171
GTGTAGG63350.015.6680871
GTACTAG36550.015.0869941
GTATTAC53950.014.9909811
GTATAGG43850.014.8109821
GTATAGA66500.014.0045611
ATACTGT121800.013.9797726
GTGTATA111050.013.9036731
CTATACT69850.013.8545764
TATATAC136400.013.8311813
ACTGATC109000.013.8231918
GTCCTAG66050.013.7289221
GTGTAGA79000.013.33978751
GTCTATA61550.013.3390311
CTGTCGC37700.013.3220849
CCTATAC53650.013.2437543
GTCCTAT52900.012.9720891
GTATAAT82450.012.9302271
Produced by FastQC (version 0.11.2)