FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30423-1-mss.3510000003c8c8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30423-1-mss.3510000003c8c8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6423212
Sequences flagged as poor quality0
Sequence length251
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA107300.018.8431571
TACACTG95950.017.23565
TATACTG66200.016.2840445
GTCGTAG6307.140267E-515.5603331
GTATATA114950.015.4571161
TCTATAC60250.015.24937253
GTGTAGG48650.015.1125551
GTCCTAG50450.015.0591341
ACTGATC106350.014.8587768
GTATAGA56900.014.6442171
TCCGTAC21050.014.5490843
GTATTAG59600.013.98080351
CTAGTAC32550.013.5487513
GTACTAG30900.013.4831051
GTGTTAG73500.013.3374291
CCGTACC19650.013.0916494
GTATTAC41350.013.0390711
CCTATAC45200.013.0091983
GTATTAT62300.012.9815151
CTATACT62900.012.8537824
Produced by FastQC (version 0.11.2)