FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30245-mss.3510000003c7c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30245-mss.3510000003c7c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5074704
Sequences flagged as poor quality0
Sequence length251
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG67850.018.4155655
ACTCGCG8258.258212E-917.8179518
CTCGCGT8651.4828402E-816.9939989
GTCCTAT37650.016.9246081
GTCCTAC27600.016.8715421
GTGTATA108600.016.6998331
GTATATA130900.016.6632861
CCCCTAT32950.015.991651
CCTATAC37450.015.701353
GTATTAG60500.015.3935391
GTGCTAC33650.015.2948231
GTACGGA13054.9112714E-1115.0241441
GCGCTAG4950.00400978114.85341551
CAGTCCG8604.4082335E-614.2440019
CTATACT56200.014.1682454
GCTATAC32100.014.1203113
CCTACAC48100.014.0076473
TATACAG77550.013.9006655
CTATGCG24000.013.78134254
GTCCTAA42100.013.6803121
Produced by FastQC (version 0.11.2)