FastQCFastQC Report
Wed 18 Nov 2015
H7NJ7BCXX_l01n01_30230-1-mss.3510000003c8bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NJ7BCXX_l01n01_30230-1-mss.3510000003c8bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6195071
Sequences flagged as poor quality0
Sequence length251
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC27000.019.055578245
TATACTG65700.018.8321365
GGATCGT24500.017.9998788
GTATATA120350.017.4119681
ACTGATC109750.016.6308658
GATCGTT28300.015.58293159
GTATAGG38000.015.479451
GTGCTAG54050.015.41739751
AGGATCG31100.015.3620027
CTAGGAC46500.015.2800823
TATACAG82700.015.2572165
GTATAGC32800.014.944591
GTCCTAC30650.014.7934381
GTATTAT67150.014.599631
CTGATCA118000.014.4299849
GTGTAGG49650.014.3155021
GTATAAT65500.014.0319431
GTATAGA58150.013.6981361
GTGTATA105800.013.6676631
CTAGTAC32450.013.5905933
Produced by FastQC (version 0.11.2)