FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_rdpm2-r.3520000003bfde.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_rdpm2-r.3520000003bfde.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14838720
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1775811.1967406892238683Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG170420.11484818097517846Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG205700.040.94756745
GGTCGCC207150.040.60621644
GCACACG518900.039.6841418
AGATAGA567000.039.2349247
GAGCACA590650.039.1438116
AGCACAC551700.039.1430217
CACGTAG155700.039.07211721
AGAGCAC595050.039.05434815
GTGGTCG217050.039.0236642
ACACGTA164850.038.909520
CACACGT395800.038.6177519
ACGTCAG157850.038.283922
CACACGA142200.038.03400419
ACACGAG137150.038.0082320
TGGTCGC222750.037.81292743
CAGATAG214800.037.74446
GATAGAT592400.037.500148
CACGTCA160050.037.41978521
GATCGAG97500.036.9862028
ACACGTC252200.036.32537520
Produced by FastQC (version 0.11.2)