FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_rdpm2-hu.3520000003bfb4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_rdpm2-hu.3520000003bfb4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13865309
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1419211.0235689662596053Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG166900.040.70407545
GCACACG263600.040.5245418
GGTCGCC168850.040.24703644
AGCACAC284850.039.3096817
AGAGCAC314300.039.1761115
GAGCACA313000.039.13080216
CACACGT196750.038.8335719
CACGTAG88300.038.0423721
GTGGTCG180100.037.9578942
TGGTCGC182550.037.30053743
CACACGA83250.036.61924419
ACACGTA94650.036.6069820
ACACGAG81250.036.46919320
ACGTCAG84650.036.12122722
ACACGTC117250.036.11357520
GGTGGTC196550.034.81564741
AGATAGA276600.034.3782927
CACGTCA88100.034.3235221
CGGTGGT205150.033.43414740
GATAGAT285800.033.2954948
Produced by FastQC (version 0.11.2)