FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_rd.3520000003beec.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_rd.3520000003beec.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11793988
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG20900.030.24884218
CACACGT20700.026.08503719
AGCACAC27200.024.97935517
GAGCACA33100.021.6819616
AGAGCAC34100.021.1120115
GGTCGCC9950.021.03252844
CGGGGAC7450.020.8320031
CGGGGCG4000.020.2432751
ACACGTC17100.019.60440320
CGGGGAG8850.018.5531561
GTCGCCG11600.018.42888545
CCCGGGG14800.015.96112216
ACCCGGG15700.015.04608815
GGGGACC8950.014.8289212
CGGGGGG3500.014.7808031
CGGGGTG7550.014.5977981
CTCGGTG15350.013.9276638
CACGTAG13400.013.76822721
ACGTCAG15650.013.51412722
GGGGGAT16100.013.2719881
Produced by FastQC (version 0.11.2)