FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_rd-hu.3520000003bf3e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_rd-hu.3520000003bf3e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17255592
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG895120.518741982309271Illumina Single End PCR Primer 1 (100% over 51bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC108300.039.97704344
GCACACG182900.039.09161418
GTCGCCG111700.038.94206645
CACACGT141050.037.0207719
AGCACAC202800.036.90812317
AGAGCAC223500.036.58960315
GAGCACA222500.036.53203216
GTGGTCG122150.035.7205742
CACGTAG63000.034.39045721
TGGTCGC128650.033.75837743
ACACGTC90500.033.5859620
ACGTCAG61650.032.4795822
ACACGTA69450.032.42745220
ACACGAG59150.031.41783320
CACACGA64100.030.28978319
GGTGGTC145900.030.02961341
CTCGGTG147000.029.94415338
AAGAGCA312550.029.06540714
CGGTGGT152900.028.71426240
CACGTCA69050.028.57512521
Produced by FastQC (version 0.11.2)