FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_q-nuc-l.3520000003bf58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_q-nuc-l.3520000003bf58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271299
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2665693.666043715160111Illumina Single End PCR Primer 1 (100% over 51bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC90760.12481951299210772Illumina Single End PCR Primer 1 (100% over 40bp)
ACACTCTTTCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG89700.1233617267011025Illumina Single End PCR Primer 1 (100% over 41bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG294900.042.22842845
GGTCGCC294400.042.10083444
GTGGTCG299150.041.41729742
TGGTCGC304250.040.5603243
CTCGGTG322950.038.5276738
TCGGTGG334100.037.3416339
TCTCGGT333650.037.29261837
GGTGGTC336950.036.77766841
ACACTCT35050.036.581381
CGGTGGT345700.036.00375440
CACTCTT37300.034.1356162
ATCTCGG370200.033.73228536
CGGGGAC4700.027.7589741
TGACTGG30650.027.6701182
GATCTCG452850.027.52608535
GTGACTG31550.027.0930251
CGCCGTA8350.026.41035341
GACTGGA34700.025.4778753
GATCGAG12300.024.6914568
GCACACG8250.024.54282618
Produced by FastQC (version 0.11.2)