FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_pm2-hu.3520000003bf71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_pm2-hu.3520000003bf71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445360
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG5294892.870581002485178Illumina Single End PCR Primer 1 (100% over 51bp)
GTGACTGGAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG208700.11314498605611385Illumina Single End PCR Primer 1 (100% over 41bp)
ACACTCTTTCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTC195600.10604292895340618Illumina Single End PCR Primer 1 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG571350.042.4370845
GGTCGCC577900.041.94808244
GCACACG533000.041.5220518
GTGGTCG593300.041.1133742
TGGTCGC593450.040.91338343
AGAGCAC623300.040.70631815
GAGCACA621950.040.6830916
AGCACAC566200.040.65643317
CACACGT432350.040.59365519
CACGTAG193850.040.19285621
GGTGGTC614350.039.7127441
ACACGTA202050.039.6638520
CGGTGGT623900.039.20044740
ACACGTC251850.038.80678620
ACGTCAG174800.038.62714822
AGATAGA511100.037.879437
CACGTCA177150.037.8729121
ACACGAG117450.037.775820
CACACGA124000.037.35751319
AAGAGCA746850.037.00258614
Produced by FastQC (version 0.11.2)