FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_mdrdpm2.3520000003bf14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_mdrdpm2.3520000003bf14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10609620
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG214250.20193937200389836Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG52900.036.3195918
GGTCGCC28750.035.45679544
GTCGCCG29750.034.11385345
AGCACAC60000.033.52099217
CACACGT44450.033.10143319
GAGCACA69050.031.66876616
AGAGCAC69700.031.4376915
ACACGTC30000.028.49780320
CACGTAG22650.027.0179121
CTCGGTG38800.026.62249638
GTGGTCG40250.025.6617342
ACACGTA24950.025.06820920
CACACGA19400.024.35333319
CGGGGCG4400.024.0269151
TCTCGGT44050.023.70521737
TGGTCGC43850.023.24704443
ACACGAG20150.022.55409420
ACGTCAG25800.022.3244922
TCGGTGG51400.020.44623639
AAGAGCA126200.020.26868414
Produced by FastQC (version 0.11.2)