FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_hu.3520000003bf9b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_hu.3520000003bf9b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5201506
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG299180.5751795729928986Illumina Single End PCR Primer 1 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG35150.039.62812445
GCACACG92700.039.4619718
GGTCGCC35350.039.4031644
AGCACAC100550.038.30500417
GAGCACA108600.037.847616
AGAGCAC110200.037.58353415
CACACGT69050.037.3060919
CACGTAG32650.036.31432321
GTGGTCG39100.035.85429442
TGGTCGC39000.035.7154343
CACACGA30900.035.67595319
ACACGTA34250.035.60321420
ACACGAG29950.035.30624420
AGATAGA104750.034.598277
ACGTCAG31050.034.2728422
ACACGTC41450.033.43541320
GATCGAG17850.033.3980068
GATAGAT108900.033.2591328
AAGAGCA136000.032.9846414
CACGTCA32300.032.94648721
Produced by FastQC (version 0.11.2)