FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n02_ddpm2.3520000003bf07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n02_ddpm2.3520000003bf07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13283740
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG303210.2282565000519432Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG78950.038.07144518
CACACGT63350.035.2653119
GGTCGCC41250.035.07696544
AGCACAC89450.035.01042617
AGAGCAC101350.034.2730115
GAGCACA101000.034.2139716
GTCGCCG42850.033.76796745
ACACGTC42300.030.26437220
CTCGGTG48550.030.08246238
CACGTAG31350.029.99910721
ACGTCAG32200.028.29895422
CACACGA29150.028.01645319
GTGGTCG52400.027.95662342
ACACGTA36500.026.99858520
ACACGAG29000.026.84342220
TCTCGGT58050.025.46953637
CACGAGA32550.024.05458321
TGGTCGC61650.023.5794943
CACGTCA40150.022.69546521
AAGAGCA180200.022.5471514
Produced by FastQC (version 0.11.2)