FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_rdpm2-r.3510000003bfd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_rdpm2-r.3510000003bfd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14838720
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1580721.065267085031593TruSeq Adapter, Index 12 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA168930.11384405123892088TruSeq Adapter, Index 12 (100% over 40bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC242600.033.71576345
CTCGTAT237850.033.53763643
TCGTATG252800.032.417744
TCTCGTA249950.030.9328142
AATCTCG254050.030.3273140
ATCTCGT257650.029.90356841
TGTAGGG38100.023.6357611
GAGTTCA137000.022.9074521
GTAGGGA40650.022.1977562
CTTGTAA1314650.021.44326845
TAATCTC371000.020.72305939
GTAATCT371950.020.6695738
AGTTCAG258250.020.3641871
TGTAATC396500.019.35560237
CCTTGTA1555600.018.09728644
GTATGCC49900.017.2259145
TTGTAAT667600.015.88211545
AGTCGGA13650.015.5035011
ACCTTGT1825750.015.40593543
CACCTTG1932950.014.54221942
Produced by FastQC (version 0.11.2)