FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_rdpm2-hu.3510000003bfb7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_rdpm2-hu.3510000003bfb7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13865309
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC1268350.9147650441832923TruSeq Adapter, Index 11 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC201800.032.48089245
CTCGTAT201200.032.06342743
CATCTCG197400.031.33558540
TCGTATG211700.031.02583344
ATCTCGT207150.029.9041541
TCTCGTA208350.029.77511842
ACATCTC277950.022.23680739
TACATCT296950.020.94244438
CTACATC301600.020.59717637
GGCTACA680300.018.8134245
GAGTTCA62350.017.9823931
AGTTCAG113100.017.6569481
GTATGCC46050.016.90655545
GCTACAT387000.016.2031236
CGGCTAC819500.015.65078544
GACGTGT248250.015.30868151
AGTCGGA13700.015.1189591
GTTCAGA140500.013.9822782
GTAGGGA19900.013.5696342
ACGGCTA957050.013.41786743
Produced by FastQC (version 0.11.2)