FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_rd.3510000003beef.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_rd.3510000003beef.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11793988
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTCAG56200.014.74292851
CAGACGT59200.013.758585
AGACGTG58350.013.5738586
TCAGACG60250.013.481464
GACGTGT63400.012.5983837
GTGCCCC25550.012.4169524
TGCCCCG25800.012.2966325
GTTCAGA69150.012.1380652
ACGTGTG66250.011.9884928
CGTGTGC66150.011.83679
CCCCGGG27150.011.68497
CCCGGGT28700.011.3674178
TTCAGAC74150.011.3487293
GAGTTCA34900.011.03171351
GTGTGCT72050.010.92987810
TCCGATC73500.010.68314519
TGTGCTC78400.010.27420511
CCGATCT76650.010.27346320
GCCCCGG31200.010.1687146
GTGCTCT80200.09.95944712
Produced by FastQC (version 0.11.2)