FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_rd-hu.3510000003bf31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_rd-hu.3510000003bf31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17255592
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC811540.47030551023691336TruSeq Adapter, Index 6 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG127700.030.92403840
CTCGTAT139150.029.80246243
CGTATGC145200.029.08749845
ATCTCGT144300.027.5693141
TCGTATG153800.027.5342544
TCTCGTA153750.026.02115642
ATATCTC202350.019.81472639
AGTTCAG107000.017.0438631
GCCAATA443950.016.63463445
AATATCT249400.016.20254938
CAATATC251250.016.06519937
GAGTTCA59550.014.858591
CGCCAAT506600.014.5730744
GTTCAGA135650.013.105242
CCAATAT314350.013.00502436
ACGCCAA572700.012.85571143
CACGCCA586750.012.46350742
TCAGACG140800.012.2090844
GACGTGT198950.012.1881721
CAGACGT141450.012.1688865
Produced by FastQC (version 0.11.2)