FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_q-nuc-l.3510000003bf5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_q-nuc-l.3510000003bf5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271299
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2405833.308666030650094TruSeq Adapter, Index 7 (100% over 50bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTC110260.15163727966626045TruSeq Adapter, Index 7 (100% over 51bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATC88870.1222202525298437TruSeq Adapter, Index 7 (100% over 39bp)
GAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCA87020.11967600287101382TruSeq Adapter, Index 7 (100% over 40bp)
GGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCT81250.11174069447563632TruSeq Adapter, Index 7 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG75230.10346156855879533TruSeq Adapter, Index 7 (100% over 51bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT336800.034.98080443
CATCTCG323050.034.93735540
CGTATGC344350.034.92560245
ATCTCGT327100.034.60796441
TCGTATG347950.034.5841244
TCTCGTA332400.034.08999342
TCATCTC355450.031.75165439
GATCATC358450.031.47876737
ATCATCT378400.029.93826938
AGATCAT397500.028.9410336
CAGATCA494700.024.18243835
GAGTTCA23750.022.5637531
AGTTCAG51100.022.4722881
AGTCGGA6200.021.426851
CCAGATC658450.018.24709934
AGACGGA9750.018.2440191
ACTCGTA16550.018.2183542
ACCAGAT665150.018.06981333
CATATCG12350.017.8550640
CACCTCG6700.017.79931440
Produced by FastQC (version 0.11.2)