FastQCFastQC Report
Wed 4 Nov 2015
H7MT5BCXX_l02n01_pm2-hu.3510000003bf74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7MT5BCXX_l02n01_pm2-hu.3510000003bf74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18445360
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC4625102.507459870666661TruSeq Adapter, Index 9 (100% over 50bp)
CTCCAGTCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGA207970.11274922256871103TruSeq Adapter, Index 9 (100% over 40bp)
GGAAAGAGTGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAG190670.10337017005902839TruSeq Adapter, Index 9 (100% over 39bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC622600.037.96961245
CTCGTAT624100.036.67064343
TCGTATG649550.036.46708744
TCTCGTA608350.035.7373742
ATCTCGT617000.035.15611641
GATCTCG642100.033.69774240
AGATCTC655200.033.01515639
CAGATCT681650.031.7204438
TCAGATC911250.023.70322437
GTATGCC88900.021.4635745
ATCAGAT1041450.021.39449736
AGTTCAG150000.021.3428751
ACTCGTA38950.021.14378442
TGTAGGG44450.020.8168331
GTAGGGA46050.020.2300722
GAACTCG41050.019.23990840
AGTCGGA26750.019.1049821
GAGTTCA73300.018.6742941
GACGTGT437100.017.5174311
AACTCGT45750.017.2633541
Produced by FastQC (version 0.11.2)